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모든 사진(2)

Key Documents

MAB3420

Sigma-Aldrich

Anti-Tau-1 Antibody, clone PC1C6

clone PC1C6, Chemicon®, from mouse

동의어(들):

Anti-Tau Antibody

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

클론

PC1C6, monoclonal

종 반응성

human, rat, bovine

포장

antibody small pack of 25 μg

제조업체/상표

Chemicon®

기술

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG2a

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

Tau, a microtubulebinding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by glycogen synthase kinase 3 (GSK 3). Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.)

특이성

Anti-Tau-1 Antibody, clone PC1C6 binds to all known electrophoretic species of tau in human, rat and bovine brain (one-dimensional SDS-PAGE). However there is some unphosphorylated bias with clone PC1C6 as it seem to recognize only dephosphorylated serine sites at 195, 198, 199, and 202 {Szendrei, et al 1993; http://www.ncbi.nlm.nih.gov/entrez/query.fcgi-cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7680727}. Also see Billingsley & Kincaid, 1997 Biochem J 323:577-591 for additional mapping information on PC1C6.

면역원

Purified denatured bovine microtubule associated proteins.

애플리케이션

Anti-Tau-1 Antibody, clone PC1C6 is an antibody against Tau-1 for use in IH & WB with more than 65 product citations.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: Bovine brain microtubule proteins purified by two cycles of assembly and disassembly (9) are dissolved in SDS-PAGE sample buffer. Five micrograms of the microtuble preparation per lane is loaded onto a 4% to 20% SDS-PAGE gradient gel along side molecular weight markers (14.3 - 200 kD). After separation by electrophoresis, the proteins are blotted onto nitrocellulose. Tau is detected as a series of 5 bands (52-68 kD) with approximately 5 ng/mL of anti-tau1.

Immunofluorescence: A 1:1000 dilution of this antibody detected Tau in mouse primary neurons. (Basnet, N., et al. (2018). Nat. Cell Biol. 20(10); 1172-1180.

Immunohistochemistry: 5 μg/mL; stains axons in tissue primarily, however in culture Tau expression is not restricted to just axons.

Optimal working dilutions must be determined by end user.

Immunohistochemistry Protocol

Dephosphorylation of tissue sections (optional)

Dephosphorylation with alkaline phosphatase is recommended for staining neurofibrillary tangles in Alzheimer′s brain tissue with anti-tau-1 (6). This treatment changes the staining pattern of anti-tau-1 to include cell bodies, dendrites and axons of neurons. In untreated samples, anti-tau-1 stains axons only.

1. Incubate tissue sections at +32°C for 2.5 hours with constant agitation in the following solution: 100 mM Tris-HCl, pH 8.0; 130 units/mL alkaline phosphatase, 1 mM PMSF, 10 μg/mL pepstatin and 10 μg/mL leupeptin.

2. Rinse sections twice, 3 min per rinse, with 100 mM Tris-HCl, pH 8.0.

Anti-tau-1 staining

1. Block non-specific binding by incubating sections in PBS containing 1% (v/v) normal animal serum, and 0.03% (w/v) Triton X-100. The animal serum should be from the same species as the secondary antibody.

2. Rinse 3 times with PBS, 3 min per rinse.

3. Incubate sections with anti-tau-1, approximately 5 μg/mL, diluted in PBS containing 1% (v/v) normal animal serum.

4. Wash with PBS, changing the solution 3 times over a 3 min period.

5. Detect with a standard secondary antibody detection system (10-13).

표적 설명

5 bands (52–68 kDa)

결합

Replaces: AB1512

물리적 형태

0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide
Format: Purified
Protein A purified

저장 및 안정성

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
Alzheimer′s brain tissue (dephosphorylation with alkaline phosphatase is recommended for staining neurofibrillary tangles in Alzheimer’s brain tissue) or human T98G glioblastoma cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Gradients of substrate-bound laminin orient axonal specification of neurons.
Dertinger, SK; Jiang, X; Li, Z; Murthy, VN; Whitesides, GM
Proceedings of the National Academy of Sciences of the USA null
Interaction of nonreceptor tyrosine-kinase Fer and p120 catenin is involved in neuronal polarization.
Lee, Seung-Hye
Molecules and Cells, 20, 256-262 (2005)
SynCAM 1 participates in axo-dendritic contact assembly and shapes neuronal growth cones.
Stagi M, Fogel AI, Biederer T
Proceedings of the National Academy of Sciences of the USA null
NMDA mediated contextual conditioning changes miRNA expression.
Kye, MJ; Neveu, P; Lee, YS; Zhou, M; Steen, JA; Sahin, M; Kosik, KS; Silva, AJ
Testing null
Ryuji X Yamada et al.
Neuroreport, 17(6), 661-665 (2006-04-11)
Controlling axon and dendrite elongation is critical in developing precise neural circuits. Using isolated cultures of dentate granule neurons, we established an experimental system that can simultaneously monitor the behaviors of axonal and dendritic outgrowth. Our previous study shows that

문서

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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