생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
MB3.6, monoclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
동형
IgG3
배송 상태
wet ice
타겟 번역 후 변형
unmodified
특이성
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
면역원
FM9 melanoma cells
애플리케이션
Anti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.
Immunohistochemistry (frozen tissue sections)
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
물리적 형태
Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our 제품 선택기 도구.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Characterization of new serum biomarkers in breast cancer using lipid microarrays.
Yoshiya Yonekubo,Ping Wu,Aimalohi Esechie,Yueqiang Zhang,Guangwei Du
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine null
Analysis of effector cells in human antibody-dependent cellular cytotoxicity with murine monoclonal antibodies.
Ortaldo, JR et al.
Journal of immunology (Baltimore, Md. : 1950), 138(10), 3566-3572 (1987)
Biosynthesis and expression of the disialoganglioside GD2, a relevant target antigen on small cell lung carcinoma for monoclonal antibody-mediated cytolysis.
Cheresh, D A, et al.
Cancer Research, 46, 5112-5118 (1986)
Suzanne M Birks et al.
Neuro-oncology, 13(9), 950-960 (2011-08-03)
The expression of ganglioside GD3, which plays crucial roles in normal brain development, decreases in adults but is upregulated in neoplastic cells, where it regulates tumor invasion and survival. Normally a buildup of GD3 induces apoptosis, but this does not
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.