MAB1949

Anti-Laminin-5 Antibody, clone P3E4

clone P3E4, Chemicon^®, from mouse

• 동의어(들): Epiligrin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: P3E4, monoclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1
• 적합성: not suitable for activity/function inhibition
• NCBI 수납 번호: NM_005562.2; NM_018891.2
• UniProt 수납 번호: Q13753
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... LAMC2(3918)

설명

일반 설명

Epiligrin, the major component of human keratinocyte extracellular matrix, serves as the preferred integrin ligand for alpha 3 beta 1 in plasma membranes and focal adhesions, and colocalizes with alpha 6 beta 4 in hemidesmosomes. {Domloge-Hultsch, et al (1992) J Clin Invest 90(4):1628-1633}. Major glycoprotein of epidermal basement membrane, consisting of three disulphide bonded subunits of 170, 145 and 135 kD. Epiligrin is the major ligand for alpha3/beta1 integrin, is particularly prominent in the lamina lucida of the skin and is absent in patients with lethal junctional epidermolysis bullosa. Today, epiligrin is identical to laminin 5, also called BM600, nicein, and kalinin. Laminin 5 (epiligrin; Carter et al, 1991 Cell 65(4):599-610) as the primary ligand in epithelial BMs that mediates both cell anchorage (adhesion without migration) via integrin a6b4 in homeostatic tissue and cell migration via integrin a3b1 in wound repair.

The laminin-5 isoform (nicein, epiligrin, and kalinin) is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands (Kallunki et al., 1992; Stahl et al., 1997). Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule (Rousselle et al., 1991 J. Cell Biol. 114: 567-576; Baker et al., 1996 J. Cell Sci. 109: 2509-2520). Laminin-5 is synthesized initially as a 460-kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix (Marinkovich et al., 1992 J. Biol. Chem. 267: 17900-17906; Vailly et al., 1994 Eur. J. Biochem. 219: 209-218; Matsui et al., 1995 J. Biol. Chem. 270: 23496-23503). The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160 kD and from 155 to 105 kD, respectively (Marinkovich et al., 1992; Vailly et al., 1994; Matsui et al., 1995). {Goldfinger, LE (1998) J Cell Biol 141(1):255-265}.

특이성

Human epiligrin (laminin 5)

면역원

Human keratinocytes

애플리케이션

Immunohistochemistry: for use on acetone fixed tissue

Immunocytochemistry

Immunoblotting: (non-reducing)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Laminin-5 Antibody, clone P3E4 is validated for use in ELISA, IP, WB, IC, IH for the detection of Laminin-5.

물리적 형태

Format: Purified

Please see datasheet for lot-specific information.

저장 및 안정성

Store at +2 to 8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable