추천 제품
생물학적 소스
goat
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human, bovine, mouse
제조업체/상표
Chemicon®
기술
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
적합성
not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... COL4A1(1282)
특이성
Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
면역원
Human and bovine placental collagen type IV
애플리케이션
Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
Dot and slot blotting: 1:100-1:500
ELISA: 1:1000-1:4000
Indirect immunohistochemistry (frozen sections only): 1:10-1:40
Immunocytochemistry 1:10-1:20
Optimal working dilutions must be determined by the end user.
ELISA: 1:1000-1:4000
Indirect immunohistochemistry (frozen sections only): 1:10-1:40
Immunocytochemistry 1:10-1:20
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
물리적 형태
Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose™ 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
ImmunoAffinity Purified
저장 및 안정성
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
J C Myers et al.
Experimental cell research, 253(2), 587-598 (1999-12-10)
Rhabdomyosarcomas are known to recapitulate some of the early events in skeletal muscle embryogenesis, and cultures derived from these tumors have been extensively used to elucidate processes associated with the differentiation of primitive mesenchymal cells. These neoplasms have also provided
Localization of AQP5 during development of the mouse submandibular salivary gland.
Larsen, HS; Aure, MH; Peters, SB; Larsen, M; Messelt, EB; Kanli Galtung, H
Journal of Molecular Histology null
Proteolytic exposure of a cryptic site within collagen type IV is required for angiogenesis and tumor growth in vivo.
Xu, J; Rodriguez, D; Petitclerc, E; Kim, JJ; Hangai, M; Moon, YS; Davis, GE; Brooks, PC; Yuen, SM
The Journal of cell biology null
Fernando Revert et al.
The American journal of pathology, 171(5), 1419-1430 (2007-10-06)
Increased expression of Goodpasture antigen-binding protein (GPBP), a protein that binds and phosphorylates basement membrane collagen, has been associated with immune complex-mediated pathogenesis. However, recent reports have questioned this biological function and proposed that GPBP serves as a cytosolic ceramide
Connective tissue growth factor induces extracellular matrix deposition in human trabecular meshwork cells.
Junglas, Benjamin, et al.
Experimental Eye Research, 88, 1065-1075 (2009)
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