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Merck
모든 사진(2)

주요 문서

AB769

Sigma-Aldrich

Anti-Collagen Type IV Antibody

Chemicon®, from goat

동의어(들):

Anti-Anti-BSVD, Anti-Anti-BSVD1, Anti-Anti-COL4A1s, Anti-Anti-PADMAL, Anti-Anti-RATOR

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

goat

Quality Level

항체 형태

affinity purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human, bovine, mouse

제조업체/상표

Chemicon®

기술

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

적합성

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... COL4A1(1282)

특이성

Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

면역원

Human and bovine placental collagen type IV

애플리케이션

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
Dot and slot blotting: 1:100-1:500

ELISA: 1:1000-1:4000

Indirect immunohistochemistry (frozen sections only): 1:10-1:40

Immunocytochemistry 1:10-1:20

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

물리적 형태

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
ImmunoAffinity Purified

저장 및 안정성

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

J C Myers et al.
Experimental cell research, 253(2), 587-598 (1999-12-10)
Rhabdomyosarcomas are known to recapitulate some of the early events in skeletal muscle embryogenesis, and cultures derived from these tumors have been extensively used to elucidate processes associated with the differentiation of primitive mesenchymal cells. These neoplasms have also provided
Localization of AQP5 during development of the mouse submandibular salivary gland.
Larsen, HS; Aure, MH; Peters, SB; Larsen, M; Messelt, EB; Kanli Galtung, H
Journal of Molecular Histology null
Proteolytic exposure of a cryptic site within collagen type IV is required for angiogenesis and tumor growth in vivo.
Xu, J; Rodriguez, D; Petitclerc, E; Kim, JJ; Hangai, M; Moon, YS; Davis, GE; Brooks, PC; Yuen, SM
The Journal of cell biology null
Fernando Revert et al.
The American journal of pathology, 171(5), 1419-1430 (2007-10-06)
Increased expression of Goodpasture antigen-binding protein (GPBP), a protein that binds and phosphorylates basement membrane collagen, has been associated with immune complex-mediated pathogenesis. However, recent reports have questioned this biological function and proposed that GPBP serves as a cytosolic ceramide
Connective tissue growth factor induces extracellular matrix deposition in human trabecular meshwork cells.
Junglas, Benjamin, et al.
Experimental Eye Research, 88, 1065-1075 (2009)

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