추천 제품
제품명
Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28, clone AF-28, Chemicon®, from mouse
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
AF-28, monoclonal
종 반응성
bovine, rat, human, pig, mouse
반응하면 안 됨
guinea pig, horse
제조업체/상표
Chemicon®
기술
ELISA: suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ACAN(176)
일반 설명
Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.
특이성
Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.
면역원
Epitope: N-terminus FFGVG neoepitopes
FFGVGGEEDC-KLH peptide
애플리케이션
Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
Inflammation & Autoimmune Mechanisms
ECM Proteins
Inflammation & Autoimmune Mechanisms
Western blot
ELISA
Optimal working dilutions must be determined by the end user.
ELISA
Optimal working dilutions must be determined by the end user.
물리적 형태
Format: Purified
Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
Protein A purified
저장 및 안정성
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Cartilage, neural tube, and brain tissue
Cartilage, neural tube, and brain tissue
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
MMP proteolysis of the human extracellular matrix protein aggrecan is mainly a process of normal turnover.
Struglics, A; Hansson, M
The Biochemical Journal null
Soluble recombinant neprilysin induces aggrecanase-mediated cleavage of aggrecan in cartilage explant cultures
Chevrier, A, et al
Archives of Biochemistry and Biophysics, 396, 178-186 (2001)
F A Mercuri et al.
The Journal of biological chemistry, 274(45), 32387-32395 (1999-11-05)
A recombinant human aggrecan G1-G2 fragment comprising amino acids Val(1)-Arg(656) has been expressed in Sf21 cells using a baculovirus expression system. The recombinant G1-G2 (rG1-G2) was purified to homogeneity by hyaluronan-Sepharose affinity chromatography followed by high performance liquid chromatography gel
Multiple functions of Osterix are required for bone growth and homeostasis in postnatal mice.
Zhou X, Zhang Z, Feng JQ, Dusevich VM, Sinha K, Zhang H, Darnay BG, de Crombrugghe B
Proceedings of the National Academy of Sciences of the USA null
The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging.
Maijenburg, MW; Kleijer, M; Vermeul, K; Mul, EP; van Alphen, FP; van der Schoot, CE; Voermans, C
Haematologica null
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