MABT110

Anti-Aggrecan (neoepitope) Antibody, clone BC-3

clone BC-3, from mouse

• 동의어(들): Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: BC-3, monoclonal
• 종 반응성: bovine, human
• 기술: immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001126
• UniProt 수납 번호: P16112
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ACAN(176)

설명

일반 설명

Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.

특이성

This antibody detectes the Args neoepitope sequence in aggrecanase-cleaved aggrecan (Tortella, M. D., et al. (1999). Science. 284(5420):1664-1666.).

면역원

Epitope: Args neoepitope sequence

Linear peptide corresponding to human Aggrecan neoepitope.

애플리케이션

Anti-Aggrecan (neoepitope) Antibody, clone BC-3 detects level of Aggrecan (neoepitope) & has been published & validated for use in Western Blotting & IHC.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blot Analysis: A representative lot detected Aggrecan neoepitope in human chondrocite cell lysate.

품질

Evaluated by Western Blot in SW1353 cell lysate.

Western Blot Analysis:1 µg/mL of this antibody detected Aggrecan neoepitope in 10 µg of SW1353 cell lysate.

표적 설명

~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
SW1353 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable