콘텐츠로 건너뛰기
Merck
모든 사진(4)

Key Documents

MAB1618

Sigma-Aldrich

Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1

clone 74.1, Chemicon®, from mouse

동의어(들):

Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1, Cytoplasmic dynein 1 intermediate chain 2, Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosoli

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

74.1, monoclonal

종 반응성

Xenopus, mouse, fish, rat, human, sheep, Drosophila, bovine

반응하면 안 됨

squid

제조업체/상표

Chemicon®

기술

immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG2b

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... DYNC1I1(1780)

일반 설명

Cytoplasmic dynein 1 intermediate chain 1 (UniProt Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) and Cytoplasmic dynein 1 intermediate chain 2 (UniProt Q0III3; also known as Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosolic, DH IC-2) are encoded by the DYNC1I1 gene (Gene ID 613724) and DYNC1I2 gene (Gene ID 526329) in bovine, respectively. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.

특이성

Not tested on chicken, nematodes or Dictystelium.

애플리케이션

Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 detects level of Dynein & has been published & validated for use in IC, IF, IP & WB.
Immunocytochemistry:
1:50-1:100 dilution from a previous lot was used. Reacts with cultured MDCK cells, NRR cells, N1E cells, and PTK-1 cells.

Immunoprecipitation:
10 μL of a previous lot per 0.5 gm tissue was used. The monoclonal cleanly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530 kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

Immunoblotting:
1:1,000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells.

Optimal working dilutions must be determined by end user.

품질

Routinely evaluated by Western Blot on A431 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected DYNEIN on 10 μg of A431 lysates.

표적 설명

~74 kDa observed.

물리적 형태

Format: Purified
Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

분석 메모

Control
HeLa cells, A431 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Hsp90-binding immunophilins link p53 to dynein during p53 transport to the nucleus.
Galigniana, MD; Harrell, JM; O'Hagen, HM; Ljungman, M; Pratt, WB
The Journal of Biological Chemistry null
Preferentially localized dynein and perinuclear dynactin associate with nuclear pore complex proteins to mediate genomic union during mammalian fertilization.
Payne, C; Rawe, V; Ramalho-Santos, J; Simerly, C; Schatten, G
Journal of Cell Science null
Evidence for glucocorticoid receptor transport on microtubules by dynein.
Harrell, JM; Murphy, PJ; Morishima, Y; Chen, H; Mansfield, JF; Galigniana, MD; Pratt, WB
The Journal of Biological Chemistry null
Evidence that the peptidylprolyl isomerase domain of the hsp90-binding immunophilin FKBP52 is involved in both dynein interaction and glucocorticoid receptor movement to the nucleus.
M D Galigniana, C Radanyi, J M Renoir, P R Housley, W B Pratt
The Journal of Biological Chemistry null
A direct interaction between cytoplasmic dynein and kinesin I may coordinate motor activity.
Ligon, LA; Tokito, M; Finklestein, JM; Grossman, FE; Holzbaur, EL
The Journal of Biological Chemistry null

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.