L7391

Anti-LIS1 antibody, Mouse monoclonal

clone LIS1-338, purified from hybridoma cell culture

• MDL number: MFCD03457563
• UNSPSC 코드: 12352203
• PubChem Substance ID: 24896475
• NACRES: NA.41
• 결합: unconjugated
• application: ARR; IP; WB
• 클론: LIS1-338, monoclonal
• 종 반응성: zebrafish, chicken, rat, human, bovine, mouse
• citations: 15
• 기술: immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using a rat brain cytosol preparation

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: LIS1-338, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen 46 kDa
• 종 반응성: zebrafish, chicken, rat, human, bovine, mouse
• 농도: ~2 mg/mL
• 기술: immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using a rat brain cytosol preparation
• 동형: IgG1
• UniProt 수납 번호: P43034
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• SMILES string: CCCCC\C=C\C\C=C\CCCCCCCC(=O)OCC(O)COP(O)(O)=O
• InChI: 1S/C21H39O7P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-21(23)27-18-20(22)19-28-29(24,25)26/h6-7,9-10,20,22H,2-5,8,11-19H2,1H3,(H2,24,25,26)/b7-6+,10-9+
• InChI key: ZQTAMPRZFOOEEP-AVQMFFATSA-N
• 유전자 정보: human ... PAFAH1B1(5048) ; mouse ... Pafah1b1(18472) ; rat ... Pafah1b1(83572)

설명

일반 설명

Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins.

The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.

특이성

By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa).

면역원

recombinant human LIS1 protein.

애플리케이션

Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.

생화학적/생리학적 작용

LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity.

The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable