HBNMAG-51K

MILLIPLEX^® Human Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay

The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.

• 동의어(들): human bone metabolic biomarkers immunoassay panel, luminex human bone metabolism multiplex assay, millipore human bone metabolic biomarkers multiplex kit
• UNSPSC 코드: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

속성

• Quality Level: 200
• 종 반응성: human
• 제조업체/상표: Milliplex^®
• assay range: accuracy: 82-97%; standard curve range: 0.24-1,000 pg/mL; (TNFα); standard curve range: 1-3,000 pg/mL; (IL-1β); standard curve range: 1-6,000 pg/mL; (ACTH & IL-6); standard curve range: 146-600,000 pg/mL; (OC); standard curve range: 24-100,000 pg/mL; (SOST); standard curve range: 37-150,000 pg/mL; (FGF-23); standard curve range: 49-200,000 pg/mL; (Leptin); standard curve range: 5-20,000 pg/mL; (DKK-1); standard curve range: 5-20,000 pg/mL; (PTH); standard curve range: 61-250,000 pg/mL; (Insulin); standard curve range: 7-30,000 pg/mL; (OPG); standard curve range: 98-400,000 pg/mL; (OPN)
• 기술: multiplexing: suitable
• 검출 방법: fluorometric (Luminex xMAP)
• 배송 상태: wet ice

설명

일반 설명

Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation. The disruption of bone metabolism results in such disease as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and bone metastases.

MILLIPLEX^® Human Bone Magnetic Bead kit is to be used for the simultaneous quantification of any or all of the following analytics in human plasma, serum or tissue/cell lysate and culture supernatant samples: Adrenocorticotropic Hormone (ACTH), Dickkopf-1 (DKK1), IL-6, Insulin, Leptin, TNFα, Osteopotegrin (OPG), Osteocalcin (OC), Osteopontin (OPN), Sclerostin (SOST), IL-1β, Parathyroid Hormone (PTH) and FGF-23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

특이성

Cross Reactivty
No cross reactivity between the antibodies for an analyte in this panel were observed.

애플리케이션

• Analytes: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteopontin (OPN), Osteoprotegerin (OPG), PTH, Sclerostin (SOST), TNFα
• Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or extracts
• Recommended Sample Dilution: 25 μL per well of d1:2 diluted serum or plasma; cell/tissue culture samples may be used neat or diluted in appropriate control medium
• Assay Run Time: Overnight (16–18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
• Research Category: Metabolism
• Research Subcategory: Bone

특징 및 장점

Design your multiplex kit by choosing available analytes within this panel.

결합

Replaces: HBN1B-51K

기타 정보

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analytes sensitivities.

법적 정보

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

안전성 정보

• 픽토그램: GHS06,GHS08,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302 + H332,H311,H317,H318,H373,H411
• 예방조치 성명서: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• 표적 기관: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects