GF174

Sonic HedgeHog (Shh) Protein, Human Recombinant

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene.

• 동의어(들): SHH, HHG-1
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: >98% (SDS-PAGE and HPLC)
• 불순물: <0.1 μg/mg protein endotoxin (<1EU/μg)
• UniProt 수납 번호: Q15465
• 배송 상태: dry ice

설명

일반 설명

Product Source: E. coli.

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoietic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

특이성

Cross Reactivty
None

애플리케이션

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

품질

Bioactivity assay: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.

물리적 형태

Product is filtered through a 0.2 micron filter before lyophilization.

저장 및 안정성

Store at -20°C for up to 6 months from date of receipt.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable