SRP3276

SDF-1 ALPHA human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• 동의어(들): CXCL12, PBSF
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 양식: lyophilized
• 효능: 20.0-80.0 ng/mL
• 분자량: 8.0 kDa
• 포장: pkg of 10 μg
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white
• UniProt 수납 번호: P48061
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... CXCL12(6387)

설명

일반 설명

SDF-1α (stromal cell-derived factor 1 α) and β are stromal derived CXC chemokines, and signal through the CXCR4 (C-X-C motif chemokine receptor). SDF1α is an isoform of CXCL12 (C-X-C motif chemokine 12, also referred to as SDF-1). The gene is mapped to human chromosome 10q11. Recombinant human SDF-1a is an 8.0kDa protein containing 68 amino acid residues.

애플리케이션

SDF-1α (stromal cell-derived factor 1 α) human has been used to study the role of SDF-1α in cell migration and survival after TGF (transforming growth factor)-β-induced EMT (epithelial–mesenchymal transition) in hepatoma cells. It has been used to study podia formation, migration, proliferation and cell-cell adhesion of human hematopoietic progenitor cells in presence of SDF-1α.

SDF-1α (stromal cell-derived factor 1 α) human has been used in the chemotaxis assay of neural crest cells (NCCs) and as a component of Dulbecco′s modified Eagle′s medium (DMEM) in migration assay of human mesenchymal stem cells (hMSCs).

생화학적/생리학적 작용

SDF-1α (stromal cell-derived factor 1 α) and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. SDF-1 is mainly expressed in the inflamed and failing myocardium. It is involved in inflammation, proliferation, tumorigenicity and metastasis. The SDF-1 proteins exert HIV (human immunodeficiency virus) suppressive activity in cells expressing the CXCR4 (C-X-C motif chemokine receptor 4). It is upregulated in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 is also involved in neuroprotection after ischemic stroke with the help of CXCR4.

물리적 형태

Lyophilized with no additives.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable