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Merck
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Key Documents

F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

동의어(들):

Ready-to-use density gradient media

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352207
NACRES:
NA.75

무균

0.2 μm filtered

Quality Level

유형

Type 400

형태

liquid

농도

20% in H2O

색상

clear colorless

solubility

water: soluble

density

1.055-1.075 g/mL

응용 분야

hematology
histology

저장 온도

2-8°C

일반 설명

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

애플리케이션

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

기타 정보

A nonionic synthetic polymer of sucrose.

법적 정보

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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프로토콜

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

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