콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

F1903

Sigma-Aldrich

Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352202
eCl@ss:
32160405
NACRES:
NA.75

생물학적 소스

human

Quality Level

형태

lyophilized

분자량

40 kDa

제조업체/상표

Chemicon®

농도

1 mg/mL

기술

cell culture | mammalian: suitable

입력

sample type epithelial cells
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

유전자 정보

human ... FN1(2335)

일반 설명

Heparin-binding 40 kDa fragment of human plasma fibronectin purified from chymotryptic digest of human plasma fibronectin. This preparation contains the CS-1 heparin-binding region of fibronectin. Heparin-binding fragment promotes cytoskeleton organization in integrin signaling. This preparation shows one major band at 40 kDa in SDS-PAGE with no higher molecular weight species present. Smaller proteolytic fragments may be present.

Human sourced raw material used in the production of this product tested negative for hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2), human T-cell lymphotropic virus (HTLV-1 and HTLV-2) and Treponema pallidum. Handle as if potentially infectious.
Product Source: Human

애플리케이션

ELISA

Affinity chromatography

Possibly for molecular mapping of fibronectin function

Cell attachment and integrin signaling assays

Optimal working dilutions must be determined by the end user.

물리적 형태

Purified by gelatin-Sepharose and heparin-Sepharose column chromatography. Lyophilized from sodium citrate buffered saline, 20mM, NaCl 150mM, pH 7.4. Reconstitute with distilled water.

저장 및 안정성

Maintain lyophilized material at -20°C for up to one year from date of receipt. After reconstitution, maintain at -20°C for up to six months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Alignment of biologically active domains in the fibronectin molecule
Ruoslahti, E. et al.
The Journal of Biological Chemistry, 256, 7277-7281 (1981)
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
Pierschbacher, M D, et al.
Cell, 26, 259-267 (1981)

문서

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

프로토콜

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

관련 콘텐츠

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.