추천 제품
Quality Level
종 반응성
human
제조업체/상표
Chemicon®
기술
activity assay: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
유전자 정보
human ... PLAU(5328)
일반 설명
The CHEMICON PAI Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of inhibition of uPA activity by PAI and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over an inhibition range of 0.05-50 units of uPA activity.
The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.
The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.
Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E.Coli-expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.
The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.
Application:
The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.
Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E. coli expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.
The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.
The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.
Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E.Coli-expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.
The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.
Application:
The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.
Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E. coli expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.
The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
애플리케이션
Research Category
Cell Structure
Cell Structure
The PAI Activity Assay Kit provides a quick, efficient & sensitive system for evaluation of inhibition of uPA activity by PAI & for screening of uPA inhibitors.
포장
96 wells
성분
uPA Standard: (Part No. 90092) One lyophilized vial, 1000 units, of uPA from human urine.
Chromogenic Substrate: (Part No. 90545) One 5 mg bottle of Tripeptide with pNA group.
Assay Buffer, 10X: (Part No. 90091) One 5 mL bottle.
PAI-1 Positive Control: (Part No. 90093) One 400 μL vial containing 20 μg of purified PAI-1 with 0.1% BSA.
Chromogenic Substrate: (Part No. 90545) One 5 mg bottle of Tripeptide with pNA group.
Assay Buffer, 10X: (Part No. 90091) One 5 mL bottle.
PAI-1 Positive Control: (Part No. 90093) One 400 μL vial containing 20 μg of purified PAI-1 with 0.1% BSA.
저장 및 안정성
Store kit materials at -20°C for up to 12 months. Store reconstituted chromogenic substrate at 2-8°C.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Byunghee Yoo et al.
Magnetic resonance in medicine, 71(3), 1221-1230 (2013-05-04)
CatalyCEST MRI compares the detection of an enzyme-responsive chemical exchange saturation transfer (CEST) agent with the detection of an unresponsive "control" CEST agent that accounts for other conditions that influence CEST. The purpose of this study was to investigate the
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