추천 제품
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Chemicon®
기술
activity assay: suitable
검출 방법
colorimetric
배송 상태
ambient
일반 설명
Introduction:
Small GTPase proteins of the Rho family are pivotal regulators of several aspects of cell behavior, such as cell motility, cell proliferation and apoptosis. They have a central role in many motile responses that involve the actin cytoskeleton and/or microtubule network, from neurite extension to phagocytosis and cancer-cell invasion. In addition, they contribute to changes in gene expression that lead to cell-cycle progression or differentiated cell responses. Rho is activated by extracellular signals such as lysophosphatidic acid (LPA). The actions of Rho are mediated by downstream Rho effectors. One of these effectors is Rho-associated protein kinase (ROCK). Two ROCK isoforms have been identified: ROCK-I (also known as ROKβ) and ROCK-II (also known as Rho Kinase and ROKα). ROCK mediates Rho signaling and reorganizes the actin cytoskeleton through phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility.
ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at threonine residue 696, which results in an increase in the phosphorylated form of the 20 kDa myosin light chain (MLC20). This threonine residue may also be phosphorylated by myotonic dystrophy protein kinase (DMPK) family kinases.
Test Principle:
Millipore’s Rho-associated Protein Kinase (ROCK) Activity Assay Kit is an enzyme immunoassay for detection of the active ROCK and DMPK family kinases. Plates are pre-coated with recombinant MYPT1, which contains a Thr696 residue that may be phosphorylated upon addition of ROCK-I, ROCK-II, or DMPK. A detection antibody that specifically detects only MYPT1 phosphorylated at Thr696 is then applied. Subsequently, an HRP-conjugated secondary detection antibody is added. The amount of phoshorylated substrate is measured by addition of the chromogenic substrate tetra-methylbenzidine (TMB), which bound HRP converts from a colorless solution to a blue solution (or yellow after the addition of stop solution). The absorbance signal at 450 nm reflects the relative amount of ROCK activity in the sample.
The ROCK assay kit is designed to determine the presence and relative amount of purified ROCK activity as well as for screening inhibitors or activators of ROCK or DMPK family kinases.
Small GTPase proteins of the Rho family are pivotal regulators of several aspects of cell behavior, such as cell motility, cell proliferation and apoptosis. They have a central role in many motile responses that involve the actin cytoskeleton and/or microtubule network, from neurite extension to phagocytosis and cancer-cell invasion. In addition, they contribute to changes in gene expression that lead to cell-cycle progression or differentiated cell responses. Rho is activated by extracellular signals such as lysophosphatidic acid (LPA). The actions of Rho are mediated by downstream Rho effectors. One of these effectors is Rho-associated protein kinase (ROCK). Two ROCK isoforms have been identified: ROCK-I (also known as ROKβ) and ROCK-II (also known as Rho Kinase and ROKα). ROCK mediates Rho signaling and reorganizes the actin cytoskeleton through phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility.
ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at threonine residue 696, which results in an increase in the phosphorylated form of the 20 kDa myosin light chain (MLC20). This threonine residue may also be phosphorylated by myotonic dystrophy protein kinase (DMPK) family kinases.
Test Principle:
Millipore’s Rho-associated Protein Kinase (ROCK) Activity Assay Kit is an enzyme immunoassay for detection of the active ROCK and DMPK family kinases. Plates are pre-coated with recombinant MYPT1, which contains a Thr696 residue that may be phosphorylated upon addition of ROCK-I, ROCK-II, or DMPK. A detection antibody that specifically detects only MYPT1 phosphorylated at Thr696 is then applied. Subsequently, an HRP-conjugated secondary detection antibody is added. The amount of phoshorylated substrate is measured by addition of the chromogenic substrate tetra-methylbenzidine (TMB), which bound HRP converts from a colorless solution to a blue solution (or yellow after the addition of stop solution). The absorbance signal at 450 nm reflects the relative amount of ROCK activity in the sample.
The ROCK assay kit is designed to determine the presence and relative amount of purified ROCK activity as well as for screening inhibitors or activators of ROCK or DMPK family kinases.
애플리케이션
Millipore’s Rho-associated Protein Kinase (ROCK) Activity Assay Kit is an enzyme immunoassay for detection of the active ROCK and DMPK family kinases. Plates are pre-coated with recombinant MYPT1, which contains a Thr696 residue that may be phosphorylated upon addition of ROCK-I, ROCK-II, or DMPK. A detection antibody that specifically detects only MYPT1 phosphorylated at Thr696 is then applied. Subsequently, an HRP-conjugated secondary detection antibody is added. The amount of phoshorylated substrate is measured by addition of the chromogenic substrate tetra-methylbenzidine (TMB), which bound HRP converts from a colorless solution to a blue solution (or yellow after the addition of stop solution). The absorbance signal at 450 nm reflects the relative amount of ROCK activity in the sample.
The ROCK assay kit is designed to determine the presence and relative amount of purified ROCK activity as well as for screening inhibitors or activators of ROCK or DMPK family kinases. It can also be used to assess the potency of pharmacological agents on ROCK or DMPK family kinases.
The ROCK assay kit is designed to determine the presence and relative amount of purified ROCK activity as well as for screening inhibitors or activators of ROCK or DMPK family kinases. It can also be used to assess the potency of pharmacological agents on ROCK or DMPK family kinases.
성분
MYPT1 Pre-coated 96-well Multistrip Plate: One 96-well multistrip plate pre-coated with MYPT1
Anti-phospho-MYPT1(Thr696) Antibody: One vial containing 10 µg of anti-phospho-MYPT1(Thr696) antibody
TMB/E Substrate: One bottle containing 12 mL
Stop Solution: One bottle containing 12 mL
TBS, 20X: One bottle containing 50 mL 20X TBS
20% Tween® 20 (v/v): One bottle containing 3 mL
Plate Sealers: Two plate covers
Assay Dilution Buffer I: One vial containing 1 mL
ATP (10mM): One vial containing 50 µL
MgCl2 (1M): One vial containing 100 µL
30% BSA: One vial containing 1 mL
Goat Anti-Rabbit IgG HRP Secondary Antibody: One vial containing 30 µL
ROCK Inhibitor Y-27632: One vial containing 150 µL
Active Rho-associated Kinase II (ROCK-II): One vial containing 10 µg
Anti-phospho-MYPT1(Thr696) Antibody: One vial containing 10 µg of anti-phospho-MYPT1(Thr696) antibody
TMB/E Substrate: One bottle containing 12 mL
Stop Solution: One bottle containing 12 mL
TBS, 20X: One bottle containing 50 mL 20X TBS
20% Tween® 20 (v/v): One bottle containing 3 mL
Plate Sealers: Two plate covers
Assay Dilution Buffer I: One vial containing 1 mL
ATP (10mM): One vial containing 50 µL
MgCl2 (1M): One vial containing 100 µL
30% BSA: One vial containing 1 mL
Goat Anti-Rabbit IgG HRP Secondary Antibody: One vial containing 30 µL
ROCK Inhibitor Y-27632: One vial containing 150 µL
Active Rho-associated Kinase II (ROCK-II): One vial containing 10 µg
저장 및 안정성
Store kit materials at specified temperatures on the label and manual; use within 4 months from date of receipt.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Met. Corr. 1 - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Karolien Hollanders et al.
Current eye research, 42(2), 260-272 (2016-07-12)
Diabetic retinopathy (DR) is characterized by an early stage of inflammation and vessel leakage, and an advanced vasoproliferative stage. Also, neurodegeneration might play an important role in disease pathogenesis. The aim of this study was to investigate the effect of
Taslim A Al-Hilal et al.
Journal of controlled release : official journal of the Controlled Release Society, 334, 237-247 (2021-04-30)
ROCK, one of the downstream regulators of Rho, controls actomyosin cytoskeleton organization, stress fiber formation, smooth muscle contraction, and cell migration. ROCK plays an important role in the pathologies of cerebral and coronary vasospasm, hypertension, cancer, and arteriosclerosis. Pharmacological-induced systemic
Ning Zhou et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(2), 701-715 (2017-11-24)
Our previous studies demonstrated that intrinsic aortic smooth muscle cell (VSMC) stiffening plays a pivotal role in aortic stiffening in aging and hypertension. However, the underlying molecular mechanisms remain largely unknown. We here hypothesized that Rho kinase (ROCK) acts as
Megan E Fox et al.
Molecular psychiatry, 25(5), 1022-1034 (2018-08-19)
Depression alters the structure and function of brain reward circuitry. Preclinical evidence suggests that medium spiny neurons (MSNs) in the nucleus accumbens (NAc) undergo structural plasticity; however, the molecular mechanism and behavioral significance is poorly understood. Here we report that
Heather L Lehman et al.
Oncotarget, 9(13), 11180-11196 (2018-03-16)
Four out of five patients diagnosed with esophageal squamous cell carcinoma (ESCC) will die within five years. This is primarily a result of the aggressive invasive potential of the disease. Our research is focused on the interplay between tumor suppressors
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.