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Merck
모든 사진(2)

Key Documents

AC111P

Sigma-Aldrich

Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate

from sheep, peroxidase conjugate

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

sheep

Quality Level

결합

peroxidase conjugate

항체 형태

purified antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

종 반응성

mouse

기술

western blot: suitable

배송 상태

ambient

타겟 번역 후 변형

unmodified

일반 설명

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc receptors which interact with sialylated Fc fragments on IgG molecules.

특이성

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reaction toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.

면역원

Purified mouse IgG.

애플리케이션

Research Category
Secondary & Control Antibodies
Research Sub Category
All
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species.
Western Blotting Analysis: Performance of a representative lot was validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of HeLa cell lysate.

품질

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: Performance of this HRP-conjugated secondary antibody (Cat No. AC111P) is validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of A431 cell lysate.

표적 설명

~150.0 kDa observed.

결합

Replaces: AP300P

물리적 형태

Ammonium Sulfate.
Purified sheep polyclonal antibody in PBS with StabilZyme® HRP Conjugate Stabilizer and 0.05% Proclin.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

StabilZyme is a registered trademark of SurModics, Inc.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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