생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
BP13, monoclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
immunofluorescence: suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TP53BP1(7158)
일반 설명
P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, and maintaining genomic stability. It is sequence specific transcription factor that plays a central role in the the response of mammalian cells to genotoxic stress. Also, 53BP1 localizes to kinetochores in mitotic cells, suggesting a potential function in mitotic checkpoint signaling.
특이성
53BP1
면역원
Recombinant human 53BP1 proteins.
애플리케이션
Detect 53BP1 also known as p53 Binding Protein 1 with Anti-53BP1 Antibody, clone BP13 (Mouse Monoclonal Antibody) validated in IF & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk.
Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.
Optimal working dilutions must be determined by the end user.
Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.
Optimal working dilutions must be determined by the end user.
물리적 형태
Format: Purified
Purified immunoglobulin. Liquid in PBS, containing sodium azide.
저장 및 안정성
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Aleksandra Lezaja et al.
Nature communications, 12(1), 3827-3827 (2021-06-24)
The paradigm that checkpoints halt cell cycle progression for genome repair has been challenged by the recent discovery of heritable DNA lesions escaping checkpoint control. How such inherited lesions affect genome function and integrity is not well understood. Here, we
Tatiana Moiseeva et al.
Nature communications, 8(1), 1392-1392 (2017-11-11)
ATR kinase activity slows replication forks and prevents origin firing in damaged cells. Here we describe proteomic analyses that identified mechanisms through which ATR kinase inhibitors induce unscheduled origin firing in undamaged cells. ATR-Chk1 inhibitor-induced origin firing is mediated by
Stefania Pellegrino et al.
Cell reports, 19(9), 1819-1831 (2017-06-01)
The bivalent histone modification reader 53BP1 accumulates around DNA double-strand breaks (DSBs), where it dictates repair pathway choice decisions by limiting DNA end resection. How this function is regulated locally and across the cell cycle to channel repair reactions toward
Kamila Burdova et al.
Cells, 8(10) (2019-10-18)
Genotoxic stress triggers a combined action of DNA repair and cell cycle checkpoint pathways. Protein phosphatase 2C delta (referred to as WIP1) is involved in timely inactivation of DNA damage response by suppressing function of p53 and other targets at
Shankar J Chinta et al.
Cell reports, 22(4), 930-940 (2018-02-02)
Exposure to the herbicide paraquat (PQ) is associated with an increased risk of idiopathic Parkinson's disease (PD). Therapies based on PQ's presumed mechanisms of action have not, however, yielded effective disease therapies. Cellular senescence is an anticancer mechanism that arrests
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