ABN2181

Anti-NCAM-L1

from rabbit

• 동의어(들): Neural cell adhesion molecule L1-like protein, L1 adhesion molecule, Cell adhesion molecule with homology to L1CAM, Chl1-like protein, Close homolog of L1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: rat, mouse
• 포장: antibody small pack of 25 μg
• 기술: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_031723
• UniProt 수납 번호: P70232
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Chl1(12661)

설명

일반 설명

Neural cell adhesion molecule L1-like protein (UniProt: P70232; also known as Cell adhesion molecule with homology to L1CAM, NCAM-L1, Chl1-like protein, Close homolog of L1) is encoded by the Chl1 (also known as Call) gene (Gene ID: in murine species. NCAM-L1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off in the mature form. It is expressed in neurons and glial cells in the brain and the spinal cord and has also been detected in the retina and the optic nerve. It is not detectable in mouse forebrain at E11 and becomes weakly detectable at E13 with highest detection at E18 to postnatal day 7. It is down-regulated at postnatal day 15 and is further reduced in four-week-old animals. NCAM-L1 is heavily N-glycosylated and contains N-linked oligosaccharides with a sulfated carbohydrate structure type HNK-1. NCAM-L1 contains an extracellular domain (aa 26-1083), a transmembrane domain (aa 1084-1104), and a cytoplasmic domain (aa 1105-1209). It also has six Ig-like C2 type domains and four fibronectin type -III domains. NCAM-L1 is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. It plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses.

특이성

This rabbit polyclonal antibody detects NCAM-L1 protein in mouse and rat.

면역원

Epitope: unknown

Purified full-length murine Neural cell adhesion molecule L1-like protein (NCAM-L1).

애플리케이션

Anti-NCAM-L1, Cat. No. ABN2181, is a highly specific rabbit polyclonal antibody that targets Neural cell adhesion molecule L1 and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected NCAM-L1 in Western Blotting applications (Schmidt, H., et. al. (2002). J Cell Biol. 159(3):489-98; Alberts, P., et. al. (2003). Mol Biol Cell. 14(10):4207-20).

Immunoprecipitation Analysis: A representative lot immunoprecipitated NCAM-L1 in Immunoprecipitation applications (Patel, K., et. al. (1991). Hybridoma. 10(4):481-91).

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NCAM-L1 in crude membrane fractions of postnatal day 10 brain extracts (Courtesy of Fritz G. Rathjen from Max-Delbrück-Center, Berlin Germany).

Immunofluorescence Analysis: A representative lot detected NCAM-L1 in Immunofluorescence applications (Ter-Avetisyan, G., et. al. (2014). J Neurosci. 34(3):737-47; Abe, P., et. al. (2015). J Neurosci. 35(38):13053-63).

품질

Evaluated by Western Blotting in Wild type & knock out P10 mouse brain extract crude membrane.

Western Blotting Analysis: 2 µg/mL of this antibody detected NCAM-L1 in crude membrane extact from brain of Wild type, but not in NCAM-L1 knock out P10 mouse.

표적 설명

~220 kDa observed; 135.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2