추천 제품
일반 설명
Aspartoacylase (EC 3.5.1.15; UniProt Q8R3P0; also known as Acy-2, Aminoacylase-2, Nur-7) is encoded by the Aspa (also known as Acy2) gene (Gene ID 11484) in murine species. Aspartoacylase catalyzes the deacetylation of N-acetylaspartate (NAA) to generate free acetate in the central nervous system (CNS). Mutations in the ASPA gene cause the autosomal recessive neurodegenerative Canavan disease (CD) that results in inadequate lipid/myelin synthesis during development. Immunohistochemical staining reveals that aspartoacylase colocalizes with the oligodendrocyte marker CC1 throughout the brain, indicating its role in myelination.
면역원
N-terminally his-tagged full-length recombinant mouse Aspa/Nur7.
애플리케이션
Immunohistochemistry Analysis: A representative lot detected Aspa/Nur7-positive oligodendrocytes in corpus callosum/external capsule using free-floating mouse brain sections (Courtesy of Dr. John R. Moffett, Uniformed Services University of the Health Sciences).
Western Blotting Analysis: A representative lot detected Aspa/Nur7 in the cytosol, but not myelin, fraction from rat brain tissue homogenate (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Immunohistochemistry Analysis: A representative lot detected Aspa/Nur7 expression pattern in various regions of rat forebrain, including corpus callosum, cerebral cortex, hippocampal commissure (hc), fimbria, and anterior commissure (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Immunohistochemistry Analysis: A representative lot detected similar Aspa/Nur7 expression pattern as CC1 in various regions of rat forebrain, including the Purkinjie & axonal fiber layers of cerebellum, corpus callosum, as well as layer 2 of primary somatosensory cortex (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Western Blotting Analysis: A representative lot detected Aspa/Nur7 in the cytosol, but not myelin, fraction from rat brain tissue homogenate (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Immunohistochemistry Analysis: A representative lot detected Aspa/Nur7 expression pattern in various regions of rat forebrain, including corpus callosum, cerebral cortex, hippocampal commissure (hc), fimbria, and anterior commissure (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Immunohistochemistry Analysis: A representative lot detected similar Aspa/Nur7 expression pattern as CC1 in various regions of rat forebrain, including the Purkinjie & axonal fiber layers of cerebellum, corpus callosum, as well as layer 2 of primary somatosensory cortex (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Aspa/Nur7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of Aspa/Nur7.
품질
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Aspa/Nur7 in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Aspa/Nur7 in 10 µg of rat brain tissue lysate.
표적 설명
~37 kDa observed
물리적 형태
Protein G Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Immunohistochemical localization of aspartoacylase in the rat central nervous system.
Madhavarao, CN; Moffett, JR; Moore, RA; Viola, RE; Namboodiri, MA; Jacobowitz, DM
The Journal of Comparative Neurology null
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