추천 제품
생물학적 소스
rabbit
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성(상동성에 의해 예측)
all
기술
ChIP: suitable
RIP: suitable
dot blot: suitable
배송 상태
wet ice
타겟 번역 후 변형
unmodified
일반 설명
N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse.
면역원
BSA-conjugated N6-methyladenosine.
애플리케이션
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in RNA containing N6-methyladenosine (Dominissini, D., et al. (2012). Nature. 485(7397):201-206.; Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).
(RNA) Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in methylated RNA containing N6-methyladenosine (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).
Dot Blot Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in a panel of modified oligonucleotides (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.; Jia, G., et al. (2011). Nat Chem Biol. 7(12):885-887.).
(RNA) Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in methylated RNA containing N6-methyladenosine (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).
Dot Blot Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in a panel of modified oligonucleotides (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.; Jia, G., et al. (2011). Nat Chem Biol. 7(12):885-887.).
Detect N6-methyladenosine (m6A) using Anti-N6-methyladenosine (m6A) Antibody validated for use in Dot Blot, Chromatin IP (ChIP) & RNA Binding Protein IP (RIP).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
품질
Evaluated by Dot Blot in oligomers containing N6-methyladenosine.
Dot Blot Analysis: 1 µg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.
Dot Blot Analysis: 1 µg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.
물리적 형태
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Karolos Douvlataniotis et al.
Science advances, 6(12), eaay3335-eaay3335 (2020-03-25)
N6-methyladenine (6mdA) is a widespread DNA modification in bacteria. More recently, 6mdA has also been characterized in mammalian DNA. However, measurements of 6mdA abundance and profiles are often very dissimilar between studies, even when performed on DNA from identical mammalian
Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons.
Meyer, Kate D, et al.
Cell, 149, 1635-1646 (2012)
Xiaoling Liu et al.
Analytical chemistry, 90(9), 5546-5551 (2018-04-14)
N6-methyladenine (6mA) is a rediscovered DNA modification in eukaryotic genomes. To explore the distribution and functions of 6mA, it is of paramount option to use immunoprecipitation to select 6mA-containing DNA fragments for genome-wide sequencing. Presumably, most of the 6mA-free fragments
Jiyu Tong et al.
Science advances, 7(18) (2021-04-30)
m6A RNA modification is implicated in multiple cellular responses. However, its function in the innate immune cells is poorly understood. Here, we identified major m6A "writers" as the top candidate genes regulating macrophage activation by LPS in an RNA binding
Guifang Jia et al.
Nature chemical biology, 7(12), 885-887 (2011-10-18)
We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of
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