ABE1302
Anti-Hec1 Antibody
from rabbit, purified by affinity chromatography
동의어(들):
Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, Hec1
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모든 사진(2)
About This Item
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human
종 반응성(상동성에 의해 예측)
rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
기술
immunocytochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NDC80(10403)
일반 설명
The protein HEC1 (highly expressed in cancer 1) is an essential component of the vital NDC80 kinetochore-associated protein complex. The NDC80 complex is required for chromosome segregation and spindle checkpoint activity. HEC1 is essential for proper kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. HEC1 is expressed by all dividing cells and is particularly high in cancers. HEC1 localizes to kinetochores from late prophase to anaphase, specifically at the outer plate of the kinetochore. HEC1 is normally phosphorylated during its active phase at multiple sites and the phosphorylation appears to tune kinetochore-microtubule binding affinity during mitosis. Moreover, recent research using small molecule inhibitors to HEC1′s kinase, Nek2, demonstrate that phosphorylation is required for proper HEC1 function.
면역원
Epitope: Nuclear localization region
KLH-conjugated linear peptide corresponding to the Nuclear localization region of human Hec1.
애플리케이션
Anti-Hec1 Antibody is an antibody against Hec1 Antibody for use in Western Blotting, Immunocytochemistry.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Hec1 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
품질
Evaluated by Western Blotting in SK-OV-3 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Hec1 in 10 µg of SK-OV-3 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Hec1 in 10 µg of SK-OV-3 cell lysate.
표적 설명
~78 kDa observed. Uncharacterized band(s) may appear in some lysates.
물리적 형태
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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