추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
Saccharomyces cerevisiae, yeast, human
제조업체/상표
Upstate®
기술
ChIP: suitable (ChIP-seq)
dot blot: suitable
multiplexing: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
acetylation (Lys14)
유전자 정보
human ... H3C1(8350)
일반 설명
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
특이성
Broad species-cross reactivity is expected.
Recognizes acetyl-histone H3 (Lys14), Mr ~17 kDa. An additional unknown protein was detected at Mr ~33 kDa.
면역원
Epitope: a.a. 9-18
Ovalbumin-conjugated, synthetic peptide (KSTGGAcKAPRK-C) corresponding to amino acids 9-18 of yeast histone H3 acetylated on lysine 14, with a C-terminal cysteine added for conjugation purposes.
애플리케이션
Anti-acetyl-Histone H3 (Lys14) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14) & has been validated in ChIP, WB, ChIP-seq, DB, Mplex.
Chromatin Immunoprecipitation (ChIP):
An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14.
Beadlyte Histone-Peptide Specificity Assay:
1:1000-1:5000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected.
An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14.
Beadlyte Histone-Peptide Specificity Assay:
1:1000-1:5000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
품질
In acid extracts from sodium butyrate treated HeLa cells.
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
표적 설명
17 kDa
결합
Replaces: 04-1044
물리적 형태
Antiserum containing 0.05% sodium azide and 30% glycerol.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
분석 메모
Control
C6 cell lysate, NIH 3T3 cell lysate, human breast carcinoma and human lung carcinoma.
C6 cell lysate, NIH 3T3 cell lysate, human breast carcinoma and human lung carcinoma.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Histone modifications on the adrenergic induction of type II deiodinase in rat pinealocytes.
Chik, CL; Price, DM; Ho, AK
Molecular and cellular endocrinology null
Zhanguo Gao et al.
Biochemical and biophysical research communications, 376(4), 793-796 (2008-10-01)
c-JUN is a major component of heterodimer transcription factor AP-1 (Activator Protein-1) that activates gene transcription in cell proliferation, inflammation and stress responses. SIRT1 (Sirtuin 1) is a histone deacetylase that controls gene transcription through modification of chromatin structure. However
ApoE is required for maintenance of the dentate gyrus neural progenitor pool.
Yang CP, Gilley JA, Zhang G, Kernie SG
Development null
Zoltan Pahi et al.
PloS one, 10(11), e0142226-e0142226 (2015-11-12)
In eukaryotes the TFIID complex is required for preinitiation complex assembly which positions RNA polymerase II around transcription start sites. On the other hand, histone acetyltransferase complexes including SAGA and ATAC, modulate transcription at several steps through modification of specific
Jan Postberg et al.
Epigenetics & chromatin, 1(1), 3-3 (2008-11-19)
In this study we exploit the unique genome organization of ciliates to characterize the biological function of histone modification patterns and chromatin plasticity for the processing of specific DNA sequences during a nuclear differentiation process. Ciliates are single-cell eukaryotes containing
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