ABC929

Anti-LC3-I/II Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_115903
• UniProt 수납 번호: Q9H492
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MAP1A(4130) , MAP1LC3A(84557)

설명

일반 설명

Microtubule-associated LC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.

특이성

This antibody detects both the non-lipidated LC3-I (non-autophagic) and lipidated LC3-II (autophagic) under autophagy conditions.

면역원

Recombinant protein corresponding to Human LC3-I/II.

애플리케이션

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected LC3 in serum-starved, chloroquine-treated HeLa cells. A punctate distribution is seen, evidence of autophagy conditions within the cell.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-LC3-I/II Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of LC3-I/II.

품질

Evaluated by Western Blotting in serum-starved, chloroquine-treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LC3-I (non-lipidated) and LC3-II (lipidated) in 10 µg of serum-starved, chloroquine-treated HeLa cell lysate.

표적 설명

~17/14 kDa observed

결합

Replaces: MABC177; MABC176

물리적 형태

Antigen Affinity Purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable