ABC232
Anti-LC3 Antibody
serum, from rabbit
동의어(들):
Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 li
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모든 사진(2)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, rat, mouse
기술
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... MAP1LC3A(84557)
일반 설명
LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound.
면역원
Linear peptide corresponding to human LC3.
애플리케이션
Anti-LC3 Antibody is an antibody against LC3 for use in Western Blotting.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blot Analysis: A 1;1,000 dilution from a representative lot detected LC3 in 10 µg of mouse embryonic fibroblast tissue lysate and MCF-7 cell lysate.
품질
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1;1,000 dilution of this antibody detected LC3 in 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: A 1;1,000 dilution of this antibody detected LC3 in 10 µg of NIH/3T3 cell lysate.
표적 설명
~16 kDa observed. LC3 can occur as a doublet around ~15 kDa, indicating the presence of both LC3-I & LC3-II. An uncharacterized band may appear at ~39 and ~55 kDa in some lysates.
결합
Replaces: MABC177; MABC176
물리적 형태
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Ribosomal protein mutations induce autophagy through S6 kinase inhibition of the insulin pathway.
Heijnen, HF; van Wijk, R; Pereboom, TC; Goos, YJ; Seinen, CW; van Oirschot et al.
PLoS Genetics null
Rosalba Siracusa et al.
International journal of molecular sciences, 22(10) (2021-06-03)
Endometriosis is a gynecological condition affecting patients in reproductive age. The aim of this paper was to assess the effects of the autophagy and mitophagy induction in a rat model of endometriosis. Endometriosis was induced by the injection of uterine
Hanna Starobinets et al.
The Journal of clinical investigation, 126(12), 4417-4429 (2016-10-25)
The rising success of cancer immunotherapy has produced immense interest in defining the clinical contexts that may benefit from this therapeutic approach. To this end, there is a need to ascertain how the therapeutic modulation of intrinsic cancer cell programs
Lyndsay Murrow et al.
Nature cell biology, 17(3), 300-310 (2015-02-17)
The ubiquitin-like molecule ATG12 is required for the early steps of autophagy. Recently, we identified ATG3, the E2-like enzyme required for LC3 lipidation during autophagy, as an ATG12 conjugation target. Here, we demonstrate that cells lacking ATG12-ATG3 have impaired basal
Jenny A Rudnick et al.
Genes & development, 35(13-14), 963-975 (2021-06-26)
Autophagy inhibitors are currently being evaluated in clinical trials for the treatment of diverse cancers, largely due to their ability to impede tumor cell survival and metabolic adaptation. More recently, there is growing interest in whether and how modulating autophagy
문서
Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.