추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
rat
종 반응성(상동성에 의해 예측)
human (based on 100% sequence homology), mouse
기술
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... FFAR2(2867)
일반 설명
Free fatty acid receptor 2 (FFAR2), also known as G-protein coupled receptor 43 (GPR43), functions as the cell membrane receptor for short chain fatty acids that ultimately stimulates G protein coupled, inhibitory (Gi) signals via the adenylate cyclase pathway. FFAR2/GPR43 is found expressed in leukocytes in the lamina propria of the small intestine mostly but some expression can be also be found in splenocytes, adipose tissue, and enteroendocrine cells of the gut. Interesting, FFAR2/GPR43 because it senses short chain fatty acids which are often key metabolites of gut microbiota, it may serve as a biological sensor to regulate the activity of regulatory T cells and the immune response in diseases such as colitis.
면역원
Epitope: Cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human FFAR2/GPR43.
애플리케이션
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-FFAR2 Antibody/GPR43 is validated for use in Western Blotting and Immunohistochemistry for the detection of FFAR2/GPR43.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected FFAR2/GPR43 in 10 µg of RAW264.7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue.
품질
Evaluated by Western Blotting in CHEM-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected FFAR2/GPR43 in 10 µg of CHEM-1 cell lysate.
표적 설명
~47 kDa observed. The calculated molecular weight of this protein is 37 kDa, but can be observed at ~47 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.
물리적 형태
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Hideaki Fujiwara et al.
Nature communications, 9(1), 3674-3674 (2018-09-12)
Microbiome-derived metabolites influence intestinal homeostasis and regulate graft-versus-host disease (GVHD), but the molecular mechanisms remain unknown. Here we show the metabolite sensor G-protein-coupled receptor 43 (GPR43) is important for attenuation of gastrointestinal GVHD in multiple clinically relevant murine models. GPR43
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