추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
mouse, human
종 반응성(상동성에 의해 예측)
rat
제조업체/상표
Chemicon®
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... MKI67(4288)
mouse ... Mki67(17345)
rat ... Mki67(291234)
일반 설명
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
특이성
Recognizes Ki-67
면역원
Synthetic peptide from human Ki-67
애플리케이션
Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB.
Immunocytochemistry:
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).
Western Blot Analysis:
A previous lot of this antibody was used in western blot.
Optimal working dilutions must be determined by the end user.
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).
Western Blot Analysis:
A previous lot of this antibody was used in western blot.
Optimal working dilutions must be determined by the end user.
품질
Immunohistochemistry(paraffin):
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
표적 설명
Predicted: 359 kDa MW may be observed at ~345 or 395 kDa
물리적 형태
Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide.
분석 메모
Control
Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.
Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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