추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, rat, mouse
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... HSP90AA1(3320)
mouse ... Hsp90Aa1(15519)
rat ... Hsp90Aa1(299331)
특이성
Reacts with a protein of 84 kD identified as heat shock protein 90beta (HSP90beta). No reactivity with HSP90alpha/HSP86.
CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.
CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.
면역원
Synthetic peptide corresponding to amino acids 2-13 from the N-terminus of mouse heat shock protein 90b.
애플리케이션
Immunoblotting: 5 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunocytochemistry
Immunoprecpitation: 10 μg/mg of protein lysate.
Optimal working dilutions must be determined by end user.
Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunocytochemistry
Immunoprecpitation: 10 μg/mg of protein lysate.
Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Protein Trafficking
Research Sub Category
Chaperones
Chaperones
This Anti-Heat Shock Protein 90β Antibody is validated for use in IP, WB, IC, IH(P) for the detection of Heat Shock Protein 90β.
물리적 형태
Format: Purified
Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.
저장 및 안정성
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
분석 메모
Control
POSITIVE CONTROL: MAD109 cells or breast carcinoma.
POSITIVE CONTROL: MAD109 cells or breast carcinoma.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE.
Putz, SM; Vogiatzi, F; Stiewe, T; Sickmann, A
Molecular Cancer null
Detection of anti-heat shock protein 90 beta (Hsp90beta) antibodies in cerebrospinal fluid.
C Cid, M Garcia-Villanueva, M Salinas, A Alcazar
Journal of Immunological Methods null
Signalling profile and antitumour activity of the novel Hsp90 inhibitor NVP-AUY922 in multiple myeloma.
Stuhmer, T; Zollinger, A; Siegmund, D; Chatterjee, M; Grella, E; Knop, S; Kortum et al.
Leukemia null
Ying-Li Wu et al.
Journal of cell science, 119(Pt 13), 2797-2806 (2006-06-15)
The involvement of telomerase in cellular immortalization and senescence has often been assessed by means of telomerase expression at the RNA level and quantification of telomerase activity by the telomeric repeat amplification protocol assay. However, these methods either neglected the
Heat shock protein 90 overexpression independently predicts inferior disease-free survival with differential expression of the alpha and beta isoforms in gastrointestinal stromal tumors.
Li, CF; Huang, WW; Wu, JM; Yu, SC; Hu, TH; Uen, YH; Tian, YF; Lin, CN; Lu, D; Fang, FM; Huang, HY
Clinical cancer research : an official journal of the American Association for Cancer Research null
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