SAB4200714

Anti-Heat Shock Protein 70 (HSP70) antibody, Mouse monoclonal

clone BRM-22, purified from hybridoma cell culture

• 동의어(들): Anti-HSP70, Anti-HSPA1A
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 항체 형태: purified from hybridoma cell culture
• Quality Level: 200
• 항체 생산 유형: primary antibodies
• 클론: BRM-22, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~70 kDa
• 종 반응성: plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse
• 농도: ~1 mg/mL
• 기술: ELISA: suitable; dot blot: suitable; immunoblotting: 0.25-0.5 μg/mL using HeLa cell line extract; immunofluorescence: suitable; immunohistochemistry: suitable
• 동형: IgG1
• UniProt 수납 번호: P0DMV8
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HSPA1A(3303) ; mouse ... HSPA1A(193740) ; rat ... HSPA1A(24472)

설명

일반 설명

Anti-heat shock protein 70 (HSP70) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the BRM-22 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified bovine brain HSP70. Monoclonal Anti-heat shock protein 70 (HSP70) specifically recognizes HSP70 from bovine, mouse, monkey, dog, human, rat, chicken,octopus, insects, plant, fish, rabbit and sea urchin origin.
HSP70 / heat shock protein family A (Hsp70) member 1A (HSPA1A), is encoded by the gene mapped to human chromosome 6p21.33. It is widely expressed in all tissues. The encoded protein is characterized with an N-terminal ATPase domain of 45kDa and a C-terminal substrate binding domain of 25kDa which is further subdivided into a β-sandwich subdomain of 15kDa and a C-terminal α-helical subdomain.

면역원

purified bovine brain HSP70

생화학적/생리학적 작용

The members of Hsp70 protein family are implicated in various cellular processes and are required for the response to environmental changes and survival. These proteins are essential constituents of the cellular network of molecular chaperones and folding catalysts. Hsp70 plays a vital role in several folding processes, such as the folding and assembly of newly synthesized proteins, refolding of misfolded and aggregated proteins. In addition, it also facilitates membrane translocation of organellar and secretory proteins and regulate the activity of regulatory proteins. Aberrations in the gene expression leads to skin color diversity.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

기타 정보

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable