AB1926
Anti-Integrin β5 Antibody, CT/cytosolic
serum, Chemicon®
동의어(들):
Anti-Anti-AA475909, Anti-Anti-AI874634, Anti-Anti-ESTM23, Anti-Anti-[b]-5, Anti-Anti-[b]5, Anti-Anti-[b]5A, Anti-Anti-[b]5B, Anti-Anti-beta-5, Anti-Anti-beta5
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모든 사진(1)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, hamster, human, rat
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ITGB5(3693)
mouse ... Itgb5(16419)
rat ... Itgb5(257645)
일반 설명
The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented.
The beta5 subunit associates with alphaV forming a receptor for vitronectin. This complex is expressed in a wide range of cell types including fibroblast and keratinocytes. It is also expressed at high levels in several tumors including lung, pancreatic and mammary carcinomas. It is not expressed in lymphblastoid cells.
The beta5 subunit associates with alphaV forming a receptor for vitronectin. This complex is expressed in a wide range of cell types including fibroblast and keratinocytes. It is also expressed at high levels in several tumors including lung, pancreatic and mammary carcinomas. It is not expressed in lymphblastoid cells.
특이성
Recognizes Integrin beta5. Specificity determined by immunoprecipitation from detergent extract of 35S methionine labeled cells. No cross-reactivity to beta1 or beta3.
면역원
Epitope: C-terminus/cytosolic
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human integrin beta 5 subunit (KTFNKFNKSYNGTVD). Note : a lysine (K) residue was added to the N-terminal of the peptide to facilitate coupling to the carrier protein.
애플리케이션
Detect Integrin β5 using this Anti-Integrin β5 Antibody, C-terminus/cytosolic validated for use in ELISA, IF, IH(P), IP, RIA & WB.
ELISA/RIA
Immunoprecipitation: 5μL of antibody is sufficient to precipitate alphaVbeta5 from
2.5x10E6 cells.
Western blot: non-reduced conditions; do not use milk in the dilution buffer.
Immunofluorescence: 1:100.
Immunohistochemistry (Acetone Fixed) 1:40-1:80
Immunohistochemistry (Paraffin Embedded) 1:200 (ABC)
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 5μL of antibody is sufficient to precipitate alphaVbeta5 from
2.5x10E6 cells.
Western blot: non-reduced conditions; do not use milk in the dilution buffer.
Immunofluorescence: 1:100.
Immunohistochemistry (Acetone Fixed) 1:40-1:80
Immunohistochemistry (Paraffin Embedded) 1:200 (ABC)
Optimal working dilutions must be determined by end user.
표적 설명
88 kDa
물리적 형태
UnPurified whole rabbit Serum containing 0.05% sodium azide as a preservative.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Individual embryonic fibroblasts express multiple beta chains in association with the alpha v integrin subunit. Loss of beta 3 expression with cell confluence.
Bates, R C, et al.
The Journal of Biological Chemistry, 266, 18593-18599 (1991)
Characterization of integrin chains in normal and neoplastic human pancreas.
Hall, P A, et al.
The Journal of Pathology, 165, 33-41 (1991)
Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
Yan, YX; Gong, YW; Guo, Y; Lv, Q; Guo, C; Zhuang, Y; Zhang, Y; Li, R; Zhang, XZ
Testing null
Integrins and extracellular matrix proteins in the human childhood and adolescent growth plate.
G Hausler, M Helmreich, S Marlovits, M Egerbacher
Calcified Tissue International null
Alpha 6.beta 1 integrin (laminin receptor) is down-regulated by tumor necrosis factor alpha and interleukin-1 beta in human endothelial cells.
Defilippi, P, et al.
The Journal of Biological Chemistry, 267, 18303-18307 (1992)
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