AB1076

Anti-phospho-ILK (Ser246) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): integrin-linked kinase, 59 kDa serine/threonine-protein kinase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, rat, mouse
• 종 반응성(상동성에 의해 예측): ox (based on 100% sequence homology)
• 기술: immunofluorescence: suitable; western blot: suitable
• NCBI 수납 번호: NP_004508
• UniProt 수납 번호: Q13418
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer246)
• 유전자 정보: human ... ILK(3611)

설명

일반 설명

ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion and migration. The kinase activity appears to be reduced following integrin activation, and overexpression of p59 ILK inhibits cellular adhesion to integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).

특이성

This antibody recognizes ILK when phosphorylated at Ser246.

면역원

Epitope: Phosphorylated Ser246

KLH-conjugated linear peptide corresponding to human ILK phosphorylated at Ser246.

애플리케이션

Detect phospho-ILK (Ser246) using this Anti-phospho-ILK (Ser246) Antibody validated for use in WB & IF.

Immunofluorescence Analysis: A previous lot was used by an independent laboratory in BT549 breast cancer cells. (Dr. Shoukat Dedhar, BC Cancer Research Centre, Vancouver, BC, Canada.)

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

품질

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ILK in 10 µg of HeLa cell lysate.

표적 설명

~54 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
HeLa cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable