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Merck
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71772

Millipore

ProteoExtract® Transmembrane Protein Extraction Kit

동의어(들):

Membrane Protein Isolation Kit

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모든 사진(1)

About This Item

UNSPSC 코드:
41106500
NACRES:
NA.77

제조업체/상표

Novagen®

저장 조건

OK to freeze

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).

애플리케이션

ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3) for extracting plasma membrane proteins

특징 및 장점

  • No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
  • Reagents are sufficient to process a total of 40 samples.

경고

Toxicity: Toxic (F)

기타 정보

Nakamura, E., et al. 2008. Cancer Sci.99, 1390.

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

픽토그램

Exclamation mark
GHS07

신호어

Warning

유해 및 위험 성명서

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

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