70181-M

NovaBlue Singles Competent Cells - Novagen

Escherichia coli, rod shaped

• 동의어(들): Competent E. coli Cells
• UNSPSC 코드: 41106202

속성

• 제품명: NovaBlue Singles Competent Cells - Novagen, NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
• 생물학적 소스: Escherichia coli
• Quality Level: 100
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 성장 모드: adherent or suspension
• 형태학: rod shaped
• 기술: microbiological culture: suitable
• 세포 형질전환: transformation efficiency: >1.5×10⁸ cfu/μg
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

일반 설명

Genotype:

endA1 hsdR17 (r_K12^– m_K12⁺) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA⁺B⁺ lacI^qZΔM15::Tn10] (Tet^R)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

NovaBlue competent cells are for routine molecular cloning applications. NovaBlue is a K-12 strain derivative that offers high transformation efficiency,facilitates plasmid stability, and allows blue/whitescreening with appropriate plasmids.

NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-l volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance).

성분

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

경고

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

DNA 프로파일

Genotype: endA1 hsdR17 (r_K12^– m_K12⁺) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA⁺B⁺ lacI^qZΔM15::Tn10] (Tet^R)

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable