553504

RAD51, His•Tag^®, Pneumocystis carinii, Recombinant, E. coli

• UNSPSC 코드: 12352202
• NACRES: NA.42

속성

• 분석: ≥95% (SDS-PAGE)
• Quality Level: 100
• 형태: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.

Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis cariniiRAD51 is equivalent to bacterial RecA and the homolog of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.

물리적 형태

In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.

기타 정보

West, S.C. 2003. Nat. Rev. Mol. Cell Biol.4, 435.
Jasin, M. 2002. Oncogene21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev.66, 630.
Daboussi, F., et al. 2002. Cell. Signal.14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res.4, 9.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable