539576

PKA, Catalytic Subunit, Bovine Heart

• 동의어(들): cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: bovine heart
• Quality Level: 100
• 분석: ≥90% (SDS-PAGE)
• 형태: liquid
• 특이 활성도: ≥20,000 units/μg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 기술: activity assay: suitable
• 적합성: suitable for molecular biology
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Research area: Cell Signaling

Native PKA catalytic subunit purified from bovine heart. Protein Kinase A (PKA) is a serine protein kinase that targets serine and threonine residues.

생화학적/생리학적 작용

Protein kinases are crucial for integrating signaling networks within eukaryotic cells. It plays an essential role in regulating proliferation and fate determination by influencing Hedgehog signaling transduction. Upon binding of cAMP to the PKA regulatory subunits, the catalytic subunit of PKA dissociates from the PKA holoenzyme and proceeds to phosphorylate target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit is capable of activation without the need for cAMP and is well-suited for in vitro studies of PKA phosphorylation with purified substrates or when introduced into intact cells or cell extracts.

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.

물리적 형태

In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Corbin, J.D., et al. 1988. Methods Enzymol159, 74.
Rannels, S.R., et al 1983. Methods Enzymol99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem.66, 253.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable