P6998

Protein Kinase A Catalytic Subunit β, Active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): PKA Catalytic Subunit β, Active human, PKA-Cβ), cAMP-dependent protein kinase
• 효소 위원회 번호: 2.7.11.11 (BRENDA, IUBMB)
• MDL number: MFCD07371063
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 재조합: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• 분석: ≥80% (SDS-PAGE)
• 형태: buffered aqueous glycerol solution
• 특이 활성도: ≥10 nmol/min·mg
• 분자량: protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa)
• UniProt 수납 번호: P22694
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PRKACB(5567)

설명

일반 설명

Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

The molecular weight of about 65kDa is confirmed by the SDS-PAGE gel. The product was generated by expressing recombinant full-length human PKAcβ2 with an additional N-terminal GST tag by baculovirus in Sf9 insect cells. The gene accession number is NM_002731. The resulting protein is 351aa cAMP-dependent protein kinase catalytic subunit beta isoform 2 (NP_002722), or according to UniProt, Isoform 1 (P22694-1).

애플리케이션

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 ^°C using a final concentration of 50 μM [³²P] ATP.

생화학적/생리학적 작용

cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

단위 정의

One unit will incorporate one nanomole of phosphate into the Crebtide (KRREILSRRPSYR) substrate per minute at 30 deg C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

물리적 형태

Supplied as a solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable