P1782

Protein kinase Cα isozyme human

≥70% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

• 동의어(들): PKCα
• MDL number: MFCD00241710
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥70% (SDS-PAGE)
• 형태: buffered aqueous glycerol solution
• 특이 활성도: ≥250 units/μg protein
• 분자량: 80-81 kDa by SDS-PAGE
• UniProt 수납 번호: P17252
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PRKCA(5578)

설명

생화학적/생리학적 작용

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP₂) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca²⁺ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca²⁺-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

단위 정의

One unit will transfer 1 pmol of phosphate from ATP to Histone H1 in 1 minute at pH 7.4 at 30 °C

물리적 형태

Supplied as a buffered aqueous glycerol solution, containing Trizma buffer, NaCl, DTT and Glutathione.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)