추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
liquid
포함
≤0.1% sodium azide as preservative
종 반응성
rat, mouse, canine, human
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
동형
IgG
배송 상태
wet ice
저장 온도
−20°C
타겟 번역 후 변형
phosphorylation (pThr180/pTyr182)
유전자 정보
human ... MAPK14(1432)
일반 설명
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr180 and Tyr182.
Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
This PhosphoDetect Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .
면역원
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
애플리케이션
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Immunocytochemistry (see comments)
경고
Toxicity: Standard Handling (A)
물리적 형태
In Dulbecco′s PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Positive Control
UV irradiated HEK293 cells or sorbitol-treated PC12 cells
UV irradiated HEK293 cells or sorbitol-treated PC12 cells
기타 정보
Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Marinissen, M.J., et al. 2001. Genes Dev.15, 535.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
Nakagami, H., et al. 2001. Diabetes50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.
Han, J., et al. 1994. Science265, 808.
Lee, J.C., et al. 1994. Nature372, 739.
Rouse, J., et al. 1994. Cell78, 1027.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
H Nakagami et al.
Diabetes, 50(6), 1472-1481 (2001-05-26)
Because high D-glucose significantly stimulates endothelial cell death, we examined the molecular mechanisms of high D-glucose-induced endothelial apoptosis. Treatment of human aortic endothelial cells with high D-glucose (25 mmol/l), but not mannitol and L-glucose, resulted in a significant decrease in
M J Marinissen et al.
Genes & development, 15(5), 535-553 (2001-03-10)
Small GTP-binding proteins of the Rho-family, Rho, Rac, and Cdc42, have been traditionally linked to the regulation of the cellular actin-based cytoskeleton. Rac and Cdc42 can also control the activity of JNK, thus acting in a molecular pathway transmitting extracellular
Douglas W Strand et al.
American journal of clinical and experimental urology, 2(3), 239-248 (2014-11-07)
Transforming Growth Factor-β (TGF-β) regulates the reactive stroma microenvironment associated with most carcinomas and mediates expression of many stromal derived factors important for tumor progression, including FGF-2 and CTGF. TGF-β is over-expressed in most carcinomas, and FGF-2 action is important
J L Wilsbacher et al.
The Journal of biological chemistry, 274(24), 16988-16994 (1999-06-08)
Mitogen-activated protein (MAP) kinases are activated with great specificity by MAP/ERK kinases (MEKs). The basis for the specific activation is not understood. In this study chimeras composed of two MAP kinases, extracellular signal-regulated protein kinase 2 and p38, were assayed
J Kaufmann et al.
Acta physiologica (Oxford, England), 213(4), 920-932 (2015-01-17)
Hypoxia and sympathetic activation are main factors in the pathogenesis of acute kidney injury (AKI). We tested the hypothesis that noradrenaline (NE) in combination with hypoxia aggravates the vasoreactivity of renal arteries after hypoxia/re-oxygenation (H/R). We tested the role of
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