407615-M

Interleukin-1β, Human, Recombinant, E. coli

• 동의어(들): Interleukin-1β, Human, Recombinant, E. coli, rhIL-1β
• CAS Number: 97599-21-8
• UNSPSC 코드: 12352202

속성

• 분석: ≥97% (SDS-PAGE)
• Quality Level: 100
• 양식: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 불순물: ≤1.0 EU/μg Endotoxin (EU/μg IL-1β)
• 저장 온도: −70°C

설명

일반 설명

Recombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. IL-1β is activated by proteolytic cleavage of the precursor molecule. Increases nitric oxide production in smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Induces nitric oxide synthase in insulin-producing cells. 1 µg of IL-1β corresponds to 1.9 x 10⁵ WHO units.

Recombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. Induces nitric oxide synthase in pancreatic and smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing.

생화학적/생리학적 작용

ED₅₀ = <12 pg/ml as measured in a cell proliferation assay using the murine T-cell line, D10.G4.1 (Symons, J.A. et al. 1987)

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.

재구성

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

기타 정보

Chun, S.Y., et al. 1995. Endocrinology136, 3120.
Kunz, D., et al. 1994. Biochem. J. 304, 337.
Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62.
Dinarello, C>A> 1991. Blood77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun.162, 357.
Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable