391334

BES

ULTROL^® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

• 동의어(들): BES, Free Acid, ULTROL^® Grade, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
• 실험식(Hill 표기법): C₆H₁₅NO₅S
• CAS Number: 10191-18-1
• Molecular Weight: 213.25
• MDL number: MFCD00007533
• UNSPSC 코드: 12161700
• NACRES: NA.25

속성

• Quality Level: 100
• 분석: ≥99% dry basis (titration)
• 형태: solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 색상: white
• pKa(20 °C): 7.12
• solubility: water: 1 mg/mL
• 양이온 미량물: heavy metals: ≤1 ppm
• 배송 상태: ambient
• 저장 온도: 15-25°C
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• InChI key: AJTVSSFTXWNIRG-UHFFFAOYSA-N

설명

일반 설명

Absorbance (100 mM, 260 nm):≤0.05.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pK_a of 7.12 at 20°C.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

경고

Toxicity: Standard Handling (A)

재구성

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

기타 정보

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable