2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• 동의어(들): Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• CAS Number: 9001-15-4
• 효소 위원회 번호: 2.7.3.2 (BRENDA, IUBMB)
• MDL number: MFCD00130876
• UNSPSC 코드: 12352202
• NACRES: NA.21

속성

• 생물학적 소스: rabbit skeletal muscle
• Quality Level: 200
• 양식: lyophilized
• 특이 활성도: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic)
• solubility: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• 외래 활성: ATPase, hexokinase, myokinase <0.001%
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

재구성

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

기타 정보

Bessman, S.P. 1987. Anal. Biochem.161, 519.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable