추천 제품
생물학적 소스
bovine heart
Quality Level
설명
Contains primarily isozyme MM
유형
Type III
형태
salt-free, lyophilized powder
특이 활성도
≥30 units/mg protein
분자량
80 kDa
저장 온도
−20°C
애플리케이션
Creatine Phosphokinase from bovine heart has been used:
- in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria
- in autoubiquitination assay
- in the preparation of premix buffer for adenylyl cyclase activity
- in the preparation of E-mix for in vitro nuclear assembly and isolation
Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves.
The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.
생화학적/생리학적 작용
Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.
주의사항
The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.
단위 정의
One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.
분석 메모
Protein determined by biuret.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Isolation, purification and characterization of creatine kinase from bovine cardiac muscle.
O S Herasymowych et al.
Biochimica et biophysica acta, 534(1), 38-47 (1978-05-24)
M S Fehrs et al.
Journal of dairy science, 64(8), 1700-1706 (1981-08-01)
Sixteen male Holstein calves in a 2 X 2 factorial design with four animals per treatment were fed 0 and 100 ppm supplemental copper from copper carbonate and 0 and 1 ppm added selenium from sodium selenite for 15 days
A C Galloway et al.
Surgery, 96(2), 435-439 (1984-08-01)
The development of myocardial ischemia is known to elicit the formation and enlargement of collateral vessels. The stimulus for these events is unknown. We have investigated the possibility that cardiac tissue releases a factor that can stimulate endothelial cell proliferation.
A general strategy for discovery of inhibitors and activators of RING and U-box E3 ligases with ubiquitin variants
Gabrielsen M, et al.
Molecular Cell, 68(2), 456-470 (2017)
Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for western blotting and scanning electron microscopy (SEM)
Allen TD, et al.
Nature Protocols, 2(5), 1173-1173 (2007)
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