추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
FMC75, monoclonal
형태
liquid
미포함
preservative
종 반응성
human, monkey
반응하면 안 됨
rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
동형
IgG1
배송 상태
wet ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
일반 설명
Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
면역원
Human
a recombinant protein consisting of human calreticulin fused to maltose binding protein
애플리케이션
Enzyme Immunoassay (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In PBS, 50% Glycerol and 0.09% Sodium Azide.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
기타 정보
Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Stephanie S Lam et al.
Nature methods, 12(1), 51-54 (2014-11-25)
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is
Ying Yin et al.
PLoS biology, 16(8), e2005140-e2005140 (2018-08-08)
Some secreted proteins that assemble into large complexes, such as extracellular matrices or hormones and enzymes in storage granules, must be kept at subaggregation concentrations during intracellular trafficking. We show surfeit locus protein 4 (Surf4) is the cargo receptor that
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