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Merck
모든 사진(1)

Key Documents

176905

Sigma-Aldrich

Anthrax Protective Antigen

from Recombinant, >90% (SDS-PAGE), lyophilized, anthrax protective antigen, Calbiochem®

동의어(들):

Anthrax PA

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.77

product name

Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis, Anthrax Protective Antigen, Bacillus anthracis, Recombinant, is the protective antigen that mediates the entry of anthrax lethal factor and edema factor into the target cell.

Quality Level

형태

lyophilized

제조업체/상표

Calbiochem®

저장 조건

OK to freeze

배송 상태

ambient

저장 온도

2-8°C

일반 설명

Recombinant, Bacillus anthracis anthrax protective antigen expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Central moiety that mediates entry of lethal factor (LF) and edema factor (EF) into the target cell. PA83 binds to the cell surface via a type I membrane receptor with a von Willebrand factor A domain, known as anthrax toxin receptor. PA83 is proteolytically activated in vivo by a furin-like protease to produce a 63 kDa protein (PA63). PA63 self-associates to form a heptameric, cation-selective, voltage-gated, membrane channel that subsequently binds to LF and/or EF. Cleavage of the 20 kDa fragment from PA83 is necessary for binding of protective antigen to LF and EF.
Recombinant, Bacillus anthracis anthrax protective antigen expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, protective antigen (PA) is the central moiety that mediates the entry of lethal factor and edema factor into the target cell. PA binds to the cell surface via a type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor. After proteolytic activation to a 63 kDa fragment, PA self-associates to form a heptameric cation-selective, voltage-gated membrane channel.

생화학적/생리학적 작용

Cell permeable: no
Primary Target
Type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor
Product does not compete with ATP.
Reversible: no

포장

yes

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.

재구성

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
Reconstitute in 100 µl sterile, distilled H₂O to yield a final concentration of 1 mg/ml.

분석 메모

Single major band by SDS-PAGE. Several smaller minor components are apparent that may represent cleavage fragments of the PA.

기타 정보

Mogridge, J., et al. 2002. Biochemistry41, 1079.
Nassi, S., et al. 2002. Biochemistry41, 1445.
Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun.286, 6.
Bradley, K.A., et al. 2001. Nature414, 225.
Brossier, F., and Mock, M. 2001. Toxicon39, 1747.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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