AL0103000
ALiCE® Cell Free Protein Expression Kit (Plant Cell Lysate)
An eukaryotic cell free expression system with unprecedented 3 mg/mL recombinant protein yield in batch mode and post-translational modifications enabled
동의어(들):
Cell-Free Expression Kit
About This Item
추천 제품
형태
liquid (aqueous solutions)
포장
pkg of 6 × 200 μL (AL0103200 (ALiCE® Midi Kit))
pkg of 6 × 50 μL (AL0103050 (ALiCE® Mini Kit))
pkg of 6 × 500 μL (AL0103500 (ALiCE® Maxi Kit))
기술
protein expression: suitable
배송 상태
dry ice
저장 온도
−70°C
일반 설명
To address this problem, ALiCE® a novel eukaryotic cell-free expression system was created by taking advantage of plant cell biology. ALiCE® offers unprecedented yields of up to 3 mg/mL of protein in batch mode. As such, it enables you to overcome the limitations of living host systems and to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
ALiCE® utilizes tobacco cell lysates, depleted of vacuoles. The system contains all factors required for in vitro transcription (RNA polymerases, NTPs) and translation reactions (ribosomes, translation initiation/elongation factors, tRNA, etc.). Using a simple protocol, both the RNA transcription and translation take place in a single tube (coupled transcription/translation).
Reactions are started by simply mixing plasmid DNA with the ALiCE® Reaction mix. The outstanding protein yields of ALiCE® are based on highly efficient protein expression over the duration of the reaction. Consequently, we recommend a reaction duration of 48 h.
ALiCE® is provided with two expression vectors, pALiCE01 and pALiCE02, which are used according to the targeting of the protein of interest. These have been specially tailored to offer excellent protein yield when used with the ALiCE® expression system. pALiCE01 is used for expression in ALiCE® cytosolic fraction. Targeting the microsomes with pALiCE02 offers the opportunity to express protein with post-translational modifications like disulfide bonds.
애플리케이션
- Target protein characterization
- Protein optimization
- Mutant screening
- Protein-protein interactions
- Expression analysis
- Protein localization analysis
- Protein structure analysis
- Post-translational modification assays
- Metabolomics
- Herbicide screening
특징 및 장점
- High-yield: Up to 3 mg/mL expressed protein
- Easy to use: One-step reaction protocol
- Fast: High yield in just 48 hours
- Flexible: Express “difficult to produce” proteins, cytotoxic proteins, etc.
성분
ALiCE® Lysate, 6 x 50 μL
Vector pALiCE01, 15 μL
Vector pALiCE02, 15 μL
ALiCE® Tube Cap, 0.6 mm hole
ALiCE® Midi Kit - AL0103200 Comprises
ALiCE® Lysate, 6 x 200 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs
ALiCE® Maxi Kit - AL0103500 Comprises
ALiCE® Lysate, 6 x 500 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs
저장 및 안정성
- ALiCE® Reaction (Lysate) mix: store at -70 °C
- Vector pALiCE01: store at -20 °C to -70 °C
- Vector pALiCE02: store at -20 °C to -70 °C
- ALiCE® Tube caps/Tube set: store at room temperature
법적 정보
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
A form of synthetic biology, cell-free protein synthesis or expression (CFPE) is a rapid method to produce recombinant proteins in solution without involving cells. Using biomolecular translation machinery extracted from a range of different cell types, researchers can express a protein of interest within a day.
프로토콜
Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.
Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.