16-157

Protein A Agarose/Salmon Sperm DNA, 2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

• 동의어(들): ChIP agarose beads, ChIP agaraose A beads, ChIP assays
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

속성

• 생물학적 소스: Staphylococcus aureus
• Quality Level: 100
• 형태: liquid
• 제조업체/상표: Upstate^®
• 기술: ChIP: suitable
• 적합성: suitable for immunoprecipitation
• 배송 상태: wet ice

설명

일반 설명

Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.

Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

애플리케이션

Suitable for use in chromatin immunoprecipitation (ChIPs) assays

품질

Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)

물리적 형태

10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable