14-429-M

MEK1 Protein, active, 10 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• 동의어(들): CFC3, MAP2K1, MAPKK1, MEL, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in E. coli
• 제품 라인: Upstate^®
• 양식: liquid
• 특이 활성도: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• 유통기한: 1 yr
• 분자량: M_w 71 kDa
• 정제법: (Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose)
• 종 반응성: human
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• solubility: soluble
• 수납 번호(accession number): (FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. ); NM_002755.2
• NCBI 수납 번호: NM_002755.2
• UniProt 수납 번호: Q02750
• 저장 온도: −70°C
• 유전자 정보: human ... MAP2K1(5604)

설명

일반 설명

Research area: Cell Signaling Protein Target: MEK1 Target Sub-Family: STE

N-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1

생화학적/생리학적 작용

Mitogen-activated protein kinase kinase 1 (MEK1) is a hydrophilic non-receptor protein kinase that shows ubiquitous expression across different cell types. It serves as a critical component of the RAS-RAF-MEK-ERK signal transduction cascade, also known as the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. This signaling pathway plays a crucial role in regulating various biological processes, including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Additionally, MEK1 protein contributes to the development of the extra-embryonic ectoderm during placentogenesis. Somatic mutations in the MEK1 gene have been observed in individuals with melanoma, a form of skin cancer.

포장

Also available in 10 μg size and in bulk

Also available in 250μg size as catalog # 14-429M - Call for pricing and availability.

품질

routinely evaluated by phosphorylation of MBP

표적 설명

MEK1

물리적 형태

Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose

저장 및 안정성

1 year at -70°C

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°C): Not applicable