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COA/COQ

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1.44154

PyroDetect Kit

Name: PyroDetect Kit
Brand: MM

Reagents and Interleukin-1ß ELISA kit for Monocyte Activation test (MAT), for Pyrogen testing

동의어(들):

Pyrogen testing kit

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About This Item

UNSPSC 코드:

41116129

NACRES:

NA.24

추천 제품

Slide 1 of 9

1 of 9

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Agency

EP Chapter 2.6.30

Quality Level

200

특징

ready-to-use

포장

pkg of 1 kit

제조업체/상표

PyroDetect

응용 분야

pharmaceutical
pyrogen testing

호환성

configured for PyroDetect Systems

배송 상태

ambient

저장 온도

2-8°C

적합성

pyrogenic bacteria

일반 설명

PyroDetect Kit is a Monocyte Activation Test (MAT) for the detection of pyrogens. This test allows the determination of the pyrogenic activity in a solution or suspension by inducing interleukin production in cell cultures with human monocytes.

애플리케이션

The PyroDetect Kit is used for the detection and quantification of the pyrogenic activity in pharmaceutical products to ensure their safety.

특징 및 장점

Ready-to-use kit
Greater product safety
Highly compatible
In vitro test with cryoblood
Reliable results
Regulatory recognition

성분

Each PyroDetect Kit contains:

Cell Culture Microplate for 96 microcultures, with lid - 1 plate
Cell Culture Medium - RPMI with L-Glutamine and HEPES - 50 mL
IL-1β Microplate - 12 strips of 8 wells each, coated with a monoclonal antibody specific for IL-1β - 1 plate
IL-1β Conjugate
Wash Buffer Concentrate
Color Reagent Α
Color Reagent Β
Stop Solution

원리

The PyroDetect System uses cryopreserved human whole blood as a source of monocytes. The response to pyrogenic substances is determined by measurement of the Interleukin-1β (IL-1β) production. The ELISA (enzyme-linked immunosorbent assay) microplate in the PyroDetect System is precoated with an antibody specific for IL-1β. Interleukin molecules present in the culture supernatant, transferred from the blood cultures, are bound to the immobilized antibody.
The unbound substances are washed away, and an enzyme-linked polyclonal antibody specific for IL-1β is added. The IL-1β bound complex is then detected in a color reaction initiated by the addition of a suitable substrate. The efficiency of IL-1β production in the pyrogen reaction is calculated from the data of a photometrical quantification of the colored product by the enzymatic reaction in an ELISA reader.