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Merck
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Key Documents

1.32450

Sigma-Aldrich

AFB staining kit for histology

for the detection of acid-fast bacteria in histological tissue

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About This Item

UNSPSC 코드:
41116130
NACRES:
NA.41

Grade

histological grade

Quality Level

형태

liquid

IVD

for in vitro diagnostic use

응용 분야

clinical testing
diagnostic assay manufacturing
hematology
histology

저장 온도

15-25°C

일반 설명

The AFB staining kit for histology - for the detection of acid-fast bacteria in histological tissue, is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a ready-to-use staining kit that when used together with other in vitro diagnostic products from our portfolio makes bacterial target structures evaluable for diagnostic purposes in histological specimen materials, for example, the lung. The AFB staining kit is based on the Ziehl-Neelsen staining method for the detection of acid-fast bacteria. The set is modified such, that heating of the carbolfuchsin solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided. The use of light green solution renders the background colour less dominant, making the acid-fast bacteria considerably more visible and thus enhancing the quality of the diagnosis. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. The AFB staining kit for histology offers a modified carbol fuchsin solution which abolishes heating (Kinyoun method). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
The package is sufficient for approx. 230 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

애플리케이션

For the detection of acid-fast bacteria in histological tissue.

분석 메모

Acid-fast bacteria: red
Background: green

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


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