1.12374

Thrombin

from bovine plasma, 50 NIH units/mg dry wt

• 동의어(들): Thrombin, Coagulation factor IIa, Fibrinogenase
• CAS Number: 9002-04-4
• UNSPSC 코드: 12352204
• EC 인덱스 번호: 232-648-7
• NACRES: NA.25

속성

• 생물학적 소스: bovine plasma
• Quality Level: 200
• 형태: solid
• 특이 활성도: 50 NIH units/mg dry wt
• 저장 온도: 2-8°C

설명

일반 설명

Thrombin, a glycosylated sodium-activated type II enzyme, is also called coagulation factor IIa. It contains two anion-binding exosites, ABE-I and ABE-II. Thrombin exists as α, β, and γ isoforms at different stages of its generation. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond.

애플리케이션

Thrombin has been used in flow cytometry analysis to investigate the basal activation level of circulating platelets in mice. It has also been used to coagulate the plasma and allow the organotypic slice to adhere to the coverslip during culturing.

생화학적/생리학적 작용

Thrombin is a serine protease critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. It mediates the conversion of inactive plasma protein fibrinogen (factor I) into biologically active fibrin. Thrombin acts as a pro-coagulant and promotes platelet aggregation. It can also produce anti-coagulant effects. It is used to treat bleeding from capillaries and small venules. During vascular injury, thrombin is crucial for controlling the macrophages, neutrophils, monocytes, SMC, and dendritic cells.

분석 메모

Appearance (colour): almost white
Appearance (description): powder
Activity (fibrinogen; pH 7.6; 37 °C): ≥ 50 U/mg

안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H315 - H319 - H334 - H335
• 예방조치 성명서: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable