1.11350

Bactident^™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

• 동의어(들): 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent
• UNSPSC 코드: 41106200
• NACRES: NA.84

속성

• Quality Level: 100
• 설명: Bactident™ E. coli 1.13303
• 형태: liquid (ready-to-use)
• 유통기한: 210 days
• 특징: ready-to-use
• 구성: (n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)
• 포장: pkg of (1 x 30 ml, Plastic bottle)
• 기술: microbe id | specific enzyme detection: suitable
• pH: <1 (20 °C in H₂O)
• 전이 온도: flash point 36 °C
• density: 0.92 g/cm³ at 20 °C
• 응용 분야: environmental; food and beverages
• 저장 온도: 2-8°C
• 적합성: Enterobacter spp.; Escherichia coli

설명

일반 설명

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

애플리케이션

Bactident^™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

결합

Replaces: 1.11350.0007

분석 메모

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

법적 정보

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS02,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H226 - H290 - H302 - H314 - H317 - H335 - H336
• 예방조치 성명서: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point (°F): 96.8 °F
• Flash Point (°C): 36 °C